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The Highly Prolific Phenotype of Lacaune Sheep Is Associated with an Ectopic Expression of the B4GALNT2 Gene within the Ovary

Figure 2

Expression of genes within the minimal FecL locus.

Total RNA from granulosa cells (GC) from small (SF, 1–3 mm), granulosa cells and theca cells (TC) from large (LF, ≥6 mm) follicles, pituitary gland (PG) and hypothalamus (HPT) were reverse-transcribed and submitted to real-time PCR analysis for quantification of B4GALNT2 and IGF2BP1 gene expression. Data are means ± SEM of relative expression to the reference gene RPL19 showed on a log scale. Asterisk indicates significant difference between means (n = 5) from non-carriers (+/+) and homozygous carriers of the FecLL mutation (L/L), **: p<0.01; ***: p<0.001.

Figure 2