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Cell-Type Specific Features of Circular RNA Expression

Figure 5

qPCR validation of relative circular RNA expression across cell type.

Total RNA from A549, AG04450 and HeLa cells was probed by qPCR using primers specific for circular isoforms of the indicated genes, and abundances were normalized using primer efficiencies estimated with a dilution series. Sequencing-based estimates are shown by comparison; sequencing values are depicted as a log fraction of total circle counts per experiment. Each qPCR and sequence value is calculated from the average of two biological replicates. Expression of LINC00340 and LPAR1 in HeLa is not detectable with the sequencing depth in this data, and these values were pinned at −60 on the log scale.

Figure 5

doi: https://doi.org/10.1371/journal.pgen.1003777.g005