Bacterial Adaptation through Loss of Function
(A,B) Percentage of members of each class for which null mutations caused significantly beneficial (A) or deleterious (B) fitness effects in at least 5%, 25%, 50%, and 75% of the 144 conditions analyzed. Colored labels on the x-axis indicate classes with a significant enrichment in null mutations (q<0.01 by a resampling test; see main text and Table S2). (C,D) Circles: total number of significant beneficial (C) or deleterious (D) null mutations present in each class across all conditions in our meta-analysis. Box plots: simulated null distribution of the same statistic for each class (see main text and Materials and Methods for details). Classes showing significant enrichments (q<0.01) relative to the corresponding null distribution have their label colored on the x-axis and the circle representing their observed value filled in green. No bar appears for the RNA class in panel A because fewer than 5% of the conditions had beneficial nulls from the RNA class; see main text for details. All classifications are from GenProtEC (http://genprotec.mbl.edu/files/geneproductfunctions.txt).