HDAC7 Is a Repressor of Myeloid Genes Whose Downregulation Is Required for Transdifferentiation of Pre-B Cells into Macrophages
Figure 8
Interaction of HDAC7 with MEF2C and its catalytic activity are essential for repression of Mac-1.
C11 cells were infected with MSCV-GFP, MSCV-GFP-HDAC7, MSCV-GFP-HDAC7(ΔMEF), MSCV-GFP-HDAC7(K86A/K88A), MSCV-GFP-HDAC7(H657A), MSCV-GFP-HDAC7(1–487) and MSCV-GFP-HDAC7(438–915) viruses and induced with β-estradiol for 48 hours. A. Cells were stained with a Mac-1 antibody and the GFP-positive fractions were gated and the results plotted. B. Percentage of Mac-1 positive cells treated with β-estradiol for 48 hours. Data are given as mean ± SEM of values obtained in three independent experiments. Statistical significance was determined by two-way ANOVA followed by Bonferrony multiple comparison test. *P<0.0001. C. RT-qPCR experiments for Itgam gene expression changes in cells treated with β-estradiol for 48 hours. Data are given as mean ± SEM of values obtained in three independent experiments. Statistical significance was determined by two-way ANOVA followed by Bonferrony multiple comparison test. *P<0.001.