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High-Resolution Transcriptome Maps Reveal Strain-Specific Regulatory Features of Multiple Campylobacter jejuni Isolates

Figure 3

SNP–dependent promoter usage in C. jejuni.

(A) dRNA–seq reads mapped to the pnk-recN operon in the SuperGenome. Red and black arrows indicate a primary (pTSS) and an internal TSS (iTSS), respectively. Corresponding alignments of the promoter region of the two TSS for pnk are shown. The iTSS within pnk is not expressed in two of the strains (81–176 and 81116) which carry a T to C exchange at the conserved “T” residue of the −10 box. (B) dRNA–seq reads mapped to the Cj0005c-Cj0004c operon, which encodes for a molydopterin containing oxidoreductase and monoheme cytochrome c. Promoter alignment of the primary TSS of Cj0005c shows disruptions in the A/T rich cyclic pattern upstream of the conserved σ70 −10 box shows in 81116. (C) Intact cells from the four C. jejuni strains were assayed for cytochrome c oxidoreductase activity in the absence (dark grey bars) and presence (light grey bars) of sulphite. The reduction of cytochrome c was measured as the increase in the absorbance at 550 nm. Strains NCTC11168, 81–176, and RM1221 showed a significant increase (p<0.0001) over basal levels (control without cells) of cytochrome c reduction when the samples were supplemented with sulphite.

Figure 3