Effectively Identifying eQTLs from Multiple Tissues by Combining Mixed Model and Meta-analytic Approaches
The chimera was crossed with a wildtype C56Bl/6J to obtain heterozygous KOs and homozygous WTs. The heterozygous KOs were backcrossed to wildtype C56Bl/6J to obtain animals that are 75% C56Bl/6J. The male and female heterozygous KOs are intercrossed and only the resulting wildtype males are used in this study. The complicated structure of the cross is due to the fact that the knockouts were designed to be used subsequently for other studies.