Drosophila cyfip Regulates Synaptic Development and Endocytosis by Suppressing Filamentous Actin Assembly
Figure 5
The excess satellite bouton formation in cyfip mutants depends on elevated BMP signaling.
(A, B) Representative synaptic boutons labeled with anti-HRP (red) and anti-pMad (red) in wild type (A) and cyfip85.1 mutants (B). pMad level was increased in cyfip85.1 mutant synapses. Scale bar, 5 µm. (C) Statistical analysis of pMad staining intensity. n≥10 for each genotype. (D–G) Representative NMJ4 synapses double-labeled with anti-HRP (red) and anti-DLG (red) from cyfip85.1 (D), madk00237/+; cyfip85.1 (E), tkv7/+; cyfip85.1 (F), and dadJ1E4/+; cyfip85.1/+ (G). Scale bar, 10 µm. (H) Statistical results for the number of satellite boutons from different genotypes. n≥15 for each genotype. * p<0.05, ** p<0.01, and *** p<0.001. Error bars indicate SEM.