Nuclear Myosin 1c Facilitates the Chromatin Modifications Required to Activate rRNA Gene Transcription and Cell Cycle Progression
Figure 1
rRNA gene occupancy of NM1, SNF2h, and actin is cell cycle-dependent.
(A) Schematic representation of the human rDNA transcription unit. (B) ChIP and qPCR on growing HeLa cells with antibodies as indicated, detected all along the human rDNA transcription unit. The values are presented as the percentages of the input signal for each primer pair. Error bars represent standard deviations. (C) Cell cycle profiles monitored by FACS analysis on growing HeLa cells, on nocodazole-blocked HeLa cells and on HeLa cells 2 h after release from the nocodazole block. (D) ChIP and qPCR on HeLa cells in early G1 at the rRNA gene promoter, 18S rDNA, 28S rDNA and IGS with antibodies as indicated below the bars. The values are presented as the percentage of the input signal for each primer pair. (E) ChIP and qPCR on mitotic HeLa cells blocked in prometaphase at the rRNA gene promoter, 18S and 28S rDNA as well as IGS with antibodies indicated below the bars. The values are presented as the percentage of the input signal for each primer pair.