Recurrent Rearrangement during Adaptive Evolution in an Interspecific Yeast Hybrid Suggests a Model for Rapid Introgression
Figure 1
CHEF analysis of randomly selected clones from F1 hybrid evolution experiments.
At generations 50, 10, 150 and 200 two clones from each of three replicate populations were chosen for electrokaryotyping. Chromosome length variants were evident in all populations: in vessel A (Replicate 1) a yellow arrow indicates an accretion in the size of one chromosome associated with the S. cerevisiae Chromosome 7+15 doublet; in vessels B and C (Replicates 2 and 3), red ovals denote absence of a 650 Kb band corresponding to S. uvarum Chromosome 11; size variation was also noted in S. uvarum Chromosome 2–4, denoted by a blue arrow. Asterisk* = S. cerevisiae Yeast Chromosome PFG Marker (New England BioLabs; Ipswich, MA; # N0345S); GSY86 = Founder S. cerevisiae×S. uvarum hybrid.