Sex-Specific Signaling in the Blood–Brain Barrier Is Required for Male Courtship in Drosophila
Figure 3
moody-α and moody-β mutants have courtship defects.
The graph shows the courtship index CI (fraction of time males spend courting during the observation period) ± SEM of the indicated genotypes, or the performance of males in a control activity assay (# of line crossings ± SEM). N = 20. Data were analyzed by ANOVA followed by Bonferroni multiple comparisons (p<0.0001). Indices that are significantly different from the controls are marked by asterisks. Δ17 is a moody null mutant with a leaky bbb. The bbb barrier defect can be rescued by the addition of either the Moody-α or Moody-β protein isoform [28]. a) In contrast, despite the intact bbb, expression of only one isoform is not sufficient for normal courtship. moody-α and moody-β mutants have a courtship defect. When both the Moody-α and Moody-β isoforms are present in moody Δ17 mutants, courtship is restored to wild-type levels. b) The activity of the mutants is not different from that of control flies.