Initiation of Genome Instability and Preneoplastic Processes through Loss of Fhit Expression
Figure 5
Fhit activates TK1 expression.
(A) Western blot analysis of TK1 and TYMS expression in siRNA transfected HEK293 cells. Western blots were performed on 5 independent experiments, and a representative blot is shown. (B) Western blot analysis of TK1 expression in siRNA transfected HEK293 cells with or with exogenous Fhit overexpression. pRcCMV expression plasmid carrying FHIT cDNA was co-transfected with FHIT siRNAs to achieve exogenous Fhit overexpression. A representative blot is shown. (C) Western blot analysis of TK1 expression in Fhit+/+ and Fhit−/− mouse kidney epithelial cells. A representative blot is shown. (D) Western blot analysis of TK1 expression in A549 cells with Fhit stably knocked down for 7–9 weeks. A representative blot is shown.