A Mimicking-of-DNA-Methylation-Patterns Pipeline for Overcoming the Restriction Barrier of Bacteria
Figure 7
Expression of GFP in N. hamburgensis X14.
The N. hamburgensis X14 strain was transformed with pWYE561 prepared from a MoDMP host. (A) Green fluorescence was observed in transformed N. hamburgensis X14 (lower row), but not in untransformed bacteria (upper row). (B) Determination of the proportion of transformed bacteria by flow cytometry analysis (lower). Bacterial culture of the N. hamburgensis X14 strain was used as a negative control.