UTX and UTY Demonstrate Histone Demethylase-Independent Function in Mouse Embryonic Development
Figure 4
UTX and UTY have essential, redundant functions in embryonic development.
(A) Schematic of mouse mutation in Uty. The Uty gene trap YUtyGT is located in intron 4. Protein annotation is illustrated at the top to denote the location of the gene trap within the Uty coding sequence. (B) Quantitative RT-PCR downstream of the gene trap (exon 15) from tail RNA of X+ YUtyGT mice demonstrates essentially no mutant RNA. (C) XUtxGT2Δ YUtyGT males (C-iii, iv) have identical phenotypes to XUtxGT2Δ XUtxGT2Δ females (Figure 3A-vi, vii). Arrowheads denote open neural tube in the head, while white and red arrows denote moderate and more severe cardiac phenotypes.