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A Combinatorial Amino Acid Code for RNA Recognition by Pentatricopeptide Repeat Proteins

Figure 1

Sedimentation Velocity Analytical Ultracentrifugation of rPPR10 and rPPR10/RNA Complexes.

(A) SV-AUC analysis of rPPR10 at 3, 6, and 12 µM. (B) SV-AUC analysis of rPPR10 (3 µM) in the presence of its 17-nt minimal RNA ligand (1.5 µM or 3 µM). The assignment of the two species at ∼5S in the top panel as either PPR10 monomer or PPR10/RNA is ambiguous, as variation in apparent S value can result when multiple species of similar abundance are in equilibrium. The root-mean-squared-deviations ranged between .007 and .013. The trace species at low S values may result from contaminating MBP and TEV protease, whereas those of larger size may represent higher order PPR10 oligomers.

Figure 1