Retrovolution: HIV–Driven Evolution of Cellular Genes and Improvement of Anticancer Drug Activation
Figure 2
The system generates genetic diversity.
Panel A. Expected relationship between the size of the target gene and the number of Retrovolution cycles required to obtain a library containing one mutated position per virion in the gene sequence, based on the mutation rate of 3.4×10−5 mutations per nt per cycle, described in the literature [3] (dashed line), and based on the rate observed experimentally in this work (solid black line). Panel B. Distribution of the mutated positions within the dCK coding sequence (783 bp) found by sequencing 40 clones at generation 16. Panel C. Position of the 12 mutations detected in the U3 region of the LTRs of all vectors sequenced starting from generation 8 (included).