Pregnancy-Induced Noncoding RNA (PINC) Associates with Polycomb Repressive Complex 2 and Regulates Mammary Epithelial Differentiation
(A–D) RIP assays were performed with mPINC overexpressing HC11 cell using antibodies to PRC2 members including, EZH2, SUZ12 and RbAp46. An MLL1 antibody was used as a negative control. (A) RT-PCR shows that overexpressed mPINC1.0, mPINC1.6 and the DCR mutant interact with PRC2 members, but not IgG or MLL1. (B–D) qPCR shows the amount of mPINC1.0 (B), mPINC1.6 (C) and DCR (D) transcript associated with each protein as a percentage of input RNA levels. Data represent mean ± SD (n = 3).