Pregnancy-Induced Noncoding RNA (PINC) Associates with Polycomb Repressive Complex 2 and Regulates Mammary Epithelial Differentiation
(A–D) RIP assays were performed with HC11 cells using antibodies to PRC2 members including, EZH2, SUZ12 and RbAp46. An MLL1 antibody was used as a negative control and an lncRNA known to interact with PRC2, Tug1, was used as a positive control. RNA was isolated from pull downs to detect associated RNAs. (A) RT-PCR shows mPINC is associated with PRC2 members, but not MLL1. (B–D) qPCR shows the amount of Tug1 (B), mPINC1.0 (C) and mPINC1.6 (D) transcript associated with each protein as a percentage of input RNA levels. Data represent mean ± SD (n = 3).