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Pregnancy-Induced Noncoding RNA (PINC) Associates with Polycomb Repressive Complex 2 and Regulates Mammary Epithelial Differentiation

Figure 7

mPINC interacts with PRC2 in HC11 cells.

(A–D) RIP assays were performed with HC11 cells using antibodies to PRC2 members including, EZH2, SUZ12 and RbAp46. An MLL1 antibody was used as a negative control and an lncRNA known to interact with PRC2, Tug1, was used as a positive control. RNA was isolated from pull downs to detect associated RNAs. (A) RT-PCR shows mPINC is associated with PRC2 members, but not MLL1. (B–D) qPCR shows the amount of Tug1 (B), mPINC1.0 (C) and mPINC1.6 (D) transcript associated with each protein as a percentage of input RNA levels. Data represent mean ± SD (n = 3).

Figure 7

doi: https://doi.org/10.1371/journal.pgen.1002840.g007