Target Gene Analysis by Microarrays and Chromatin Immunoprecipitation Identifies HEY Proteins as Highly Redundant bHLH Repressors
Figure 1
HEY target gene validation and gene ontology analysis.
(A) Number of down- and upregulated genes from microarray analysis of HEK293 cells after induction of HEY1 or HEY2. (B) Real-time RT-PCR validation of gene regulation by inducible HEY1, HEY2, HEYL expression and upon HEY1 knock-down (shRNA). Shown is the fold change between the induced and uninduced state. Note that microarray probes and real-time PCR primers only recognize the endogenous HEY transcripts. In the overexpression situation total HEY1, HEY2 and HEYL were >220 fold induced compared to endogenous levels. (C) GO term analysis was performed to identify biological processes enriched among HEY1 and HEY2 regulated genes (≥1.3 fold). The top 14 annotation clusters are shown according to their cluster enrichment score [−lg(mean p-value)]. Cluster names are based on enriched GO annotations. “U” indicates the corresponding value for upregulated and “D” for down regulated genes.