KATNAL1 Regulation of Sertoli Cell Microtubule Dynamics Is Essential for Spermiogenesis and Male Fertility
Figure 4
The identified mutation in Katnal11H results in loss of function.
(a, b) Transduction of HT1080 cells with lentiviral particles containing the WT cDNA of Katnal1 under the control of the CMV promoter results in a significant increase in numbers of cells arrested in mitosis (arrows) 48 hours post-transduction. Conversely, numbers of cells in mitotic arrest does not differ from controls following transduction of the mutant Katnal11H cDNA. (c) The arrest of cells during mitosis is followed by a significant increase in cell death in cells transduced with WT but not mutated Katnal11H. Together these data confirm that the observed mutation in Katnal11H generates a loss of function allele. Bar = 50 µm (*** = p<0.001).