Identification of Genes That Promote or Antagonize Somatic Homolog Pairing Using a High-Throughput FISH–Based Screen
a, Representative FISH images are shown for RNAi knockdown of candidate pairing promoters (slmb, lin19, shtd, klp61f, dhc64C, mcph1, borr), where the number of FISH signals per nucleus is increased compared to control. The percentage of single-signal nuclei is noted for both 359 and dodeca. n denotes number of nuclei scored. Scale bars equal 5 µm. b, Relative frequencies of interhomolog distances (unpaired = two signals >1.0 µm apart) based on dodeca FISH ± SD for three tests. dsRNA targets are either grouped based on known interactions (SCF, APC, CPC) or localization patterns of the proteins they encode (MTOC). All significantly reduced the percentage of paired nuclei compared to control (P<0.05). c, Chromosomal targets of euchromatic FISH probes 16E and 28B and graph displaying the percentage of single-signal nuclei ± SD following RNAi. Asterisks denote a significant reduction from control (P<0.05). A minimum number of 100 nuclei were scored for each dsRNA.