Context-Dependent Dual Role of SKI8 Homologs in mRNA Synthesis and Turnover
Figure 4
Phenotypes of the Atski2 mutant and vip3zwg rescued by transgenic ScSki8 expression.
(A) A phylogenetic tree of SKI2 homologs from various eukaryotic species including AtSKI2. (B) Comparison of adult wild type Col-0 plants (right) and Atski2 mutants (left). (C) Close-up of an Atski2 mutant. (D–E) Comparison between wild type, Atski2 and vip3zwg mutant flowers at anthesis. (F) Semi-quantitative RT-PCR (increasing number of amplification cycles) of transgenic ScSki8 expression in vip3zwg plants as compared to the elongation factor 1 gene (EF1). (G–H) Illustration of the rescue of dwarf (G) and flower development (H) phenotypes by transgenic expression of ScSki8 in vip3zwg mutants. Size bars are 1 cm (B, C, G) and 1 mm (D, E, H).