Probing the Informational and Regulatory Plasticity of a Transcription Factor DNA–Binding Domain
The relative binding affinity of each mutant to each binding site () was calculated using the models presented in Figure 5. This was plotted against the of binding for each sequence as determined by the difference in binding energy between that sequence and the highest affinity site (kJ/mol). A linear regression line was fit to sites with an bits (blue sequences) and sites bits (red sequences). The intercept of these lines were used to approximate the boundary between specifically and non-specifically bound sites and the corresponding values are reported in Table 2. values for each regression line are given in the upper right hand corner in the same color.