Repression of a Potassium Channel by Nuclear Hormone Receptor and TGF-β Signaling Modulates Insulin Signaling in Caenorhabditis elegans
Figure 1
Interaction of NHR-69 with DAF-8 and expression pattern of nhr-69p::nhr-69::gfp.
(A) In vivo co-immunoprecipitation was performed with anti-DAF-8 antibody from wild-type worms and nhr-69p::nhr-69::gfp expressing animals, followed by immunoblot with anti-GFP antibody to detect NHR-69::GFP. (B) In vitro GST pull-down assay between NHR-69 and SMAD proteins (DAF-8, DAF-14, or DAF-3). C–N show DIC and fluorescence images of developing embryos (C–F) and of adults (G–N) expressing nhr-69p::nhr-69::gfp. (C and D) Expression at the E16 stage of intestinal precursor cells in embryos. (E and F) Expression at the comma stage. (G and H) Expression in ASI neurons (arrows). (I and J) Hypodermal expression. (K and L) Intestinal expression. (M and N) Expression in tail neurons. IntP; Intestinal precursor cells, HypN; Hypodermal nucleus, IntN; Intestinal nucleus, TN; Tail neurons.