Cell Lineage Analysis of the Mammalian Female Germline
a) Two modes of expansion-migrations of a small pool of progenitors which become lineage restricted during the epiblast stage. In a spatially coherent mode (1) progenies remain physically close together as they expand in numbers and thus separation into the two ovaries creates two distinct populations. In a spatially incoherent mode (2) progenies physically mix and thus separation into the two ovaries result in equal samples of the original progenitors. b) Reconstructed cell lineage trees show that GV oocytes from large antral follicles of the right (blue) or left (red) ovaries do not form distinct clusters. As a positive control cumulus cells sampled from two follicles – one in the right ovary (green) and one in the left (purple) do form distinct clusters, as expected given the previously estimated small number of progenitors of the epithelial population of a given follicle.