Adaptive Evolution of the Lactose Utilization Network in Experimentally Evolved Populations of Escherichia coli
Sequence alignments for evolved clones relative to the ancestor are shown for the lacI mutation hotspot region (left) and the primary lacO1 operator (right). Clones were chosen that represent the diversity in lac genotypes within each population. Colored boxes by sequences indicate the inducer response type for each clone. Bimodal is shown as green, constitutive is shown as blue and lower threshold response is shown as purple. No sequence was obtained for the G/L5 clones. Sequence alignments are not shown for Glu clones since all have wt lac regulatory sequences.