The Drosophila melanogaster Seminal Fluid Protease “Seminase” Regulates Proteolytic and Post-Mating Reproductive Processes
(A) Western blots probed for the proteins indicated to the left of blots. Lanes 1 and 2 contain 20 seminal receptacles (SR) dissected from females mated to control (+) or seminase knockdown (−) males at 2 hours ASM for SP, CG1656, and CG1652, and 1 hour ASM for CG9997. CG9997 was probed on a separate blot from CG1652, CG1656, and sex peptide. Lanes 4 and 5 contain 4 reproductive tracts (RT; not including ovaries or sperm storage organs, SSOs) from a subset of the females dissected in SR lanes. Tubulin is shown as a loading control for both gels. (B) Western blot probed for CG17575. Lane 1: male accessory glands (AG). Lanes 2 and 3 contain 4 RTs (excluding ovaries) from females mated to control (+) or seminase knockdown (−) males at 45 minutes ASM. Acp62F is shown as a loading control. (C) Western blot probed for seminase. Lane 1 contains female RT excluding ovaries and SSOs from wildtype mated females. Lane 2 contains 80 SR from wildtype mated females.