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The Drosophila melanogaster Seminal Fluid Protease “Seminase” Regulates Proteolytic and Post-Mating Reproductive Processes

Figure 3

Females mated to seminase knockdown males lay fewer eggs.

(A) The average number of eggs laid per female in a given treatment over 10 days. Seminase Line 1: Control N = 55, RNAi N = 59; CG4815: Control N = 20, RNAi N = 18. (B) Hatchability data for the corresponding experiments in (A). Hatchability is defined as the proportion of eggs that yielded adult progeny. CG4815 (a serine protease SFP) is included as a control for strain background. (A),(B) Asterisks indicate p<0.0001. (C) The data from (A) plotted as average number of eggs laid by females in each group on individual days of the experiment. (D) CG4815 egg laying data plotted as in (C). Asterisks indicate level of significance after Bonferroni correction (*p<0.05, **p<0.01, ***p<0.0001). Error bars indicate standard error of the raw data pooled over experiments.

Figure 3