Functional Specialization of the Plant miR396 Regulatory Network through Distinct MicroRNA–Target Interactions
Figure 3
(A) Scheme of the bHLH74 locus showing the T-DNA insertion corresponding to the GABI-Kat 720G11 line. Arrows indicate the pairs of primers (1–2 or 3–4, see also Table S7) used to quantify bHLH74 transcript levels by RT-qPCR. (B) bHLH74 transcript levels in wt and bhlh74-1 (GABI-Kat 720G11) seedlings (12–day old), using pairs of primers shown in (A). Data shown are mean ± SEM of 3 biological replicates; n.d.: not detected. Asterisks indicate significant differences between mutant and wild-type plants, as determined by ANOVA (P<0.05). (C) Number of branching points (NBP) in fully-expanded first leaves of wt, bhlh74-1 and bHLH74:rbHLH74 (lines #18 and #8) plants. Bars marked with different letters are significantly different as determined by ANOVA and Duncan's multiple range test (P<0.05). (D) Fully-expanded leaves (#1) from wt, bhlh74-1 and bHLH74:rbHLH74 (lines #18 and #8) plants. Red dots highlight branching points.