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Physiological IRE-1-XBP-1 and PEK-1 Signaling in Caenorhabditis elegans Larval Development and Immunity

Figure 5

XBP-1 and PEK-1 each protect against elevated physiological temperature and immune activity.

(A) Development of indicated mutants from eggs to the L4 larval stage or older after 4 d at 16°C on P. aeruginosa PA14. Values represent mean ± s.d. from 1 of 2 representative experiments (n = 4 plates with 20–50 eggs each, ***P<0.001, one-way ANOVA with Bonferroni post test). (B) Development of xbp-1(tm2482); pek-1(ok275) and xbp-1(tm2482); pmk-1(km25); pek-1(ok275) mutants from eggs on E. coli OP50. Values represent average fraction of eggs developed to the indicated stage ± s.e.m. (n = 2 independent experiments, **P<0.01, ***P<0.001, two-way ANOVA with Bonferroni post test). (C) Development of indicated mutants from eggs to the L4 larval stage or older after 2 d at 27°C on E. coli OP50. Values represent mean ± s.e.m. (n = 3 independent experiments, ***P<0.001, one-way ANOVA with Bonferroni post test). (D) Development of indicated mutants from eggs to the L4 larval stage or older after 2 d at 27°C on P. aeruginosa PA14. Values represent mean ± s.d. from 1 of 2 representative experiments (n = 3–4 plates with 20–60 eggs each, ***P<0.001, one-way ANOVA with Bonferroni post test).

Figure 5

doi: https://doi.org/10.1371/journal.pgen.1002391.g005