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Physiological IRE-1-XBP-1 and PEK-1 Signaling in Caenorhabditis elegans Larval Development and Immunity

Figure 3

Pathogen-induced immune activation exacerbates ER stress levels in XBP-1 deficiency in C. elegans.

Quantitative real-time PCR measurements of levels of total and spliced xbp-1 mRNA in the indicated strains grown from synchronized L1s for 23 h at 20°C and then shifted to plates with or without P. aeruginosa PA14 at 25°C for (A) 4 h or (B) 11 h. Values represent fold change ± s.e.m. (n = 2 independent experiments, ***P<0.001, two-way ANOVA with Bonferroni post test). The WT strain exposed to either treatment was normalized to WT without P. aeruginosa; all other strains were normalized to smg-2(qd101) without P. aeruginosa.

Figure 3