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Physiological IRE-1-XBP-1 and PEK-1 Signaling in Caenorhabditis elegans Larval Development and Immunity

Figure 2

XBP-1 deficiency increases PEK-1 dependent phosphorylation of eIF2α.

(A) PEK-1 dependent phosphorylation of eIF2α is induced by high dose (50 ug/ml) tunicamycin. Western blot of P-eIF2α, total eIF2α and tubulin in WT and pek-1(ok275) strains grown to the L4 stage and then shifted to plates with or without tunicamycin (50 µg/mL) for 4 hours. (B) PEK-1 dependent phosphorylation of eIF2α is induced by XBP-1 deficiency. Western blot of P-eIF2α, total eIF2α and tubulin in WT, xbp-1(tm2482), xbp-1(tm2482); pek-1(ok275) and pek-1(ok275) strains grown to the L4 stage.

Figure 2

doi: https://doi.org/10.1371/journal.pgen.1002391.g002