Temporal Dynamics of Host Molecular Responses Differentiate Symptomatic and Asymptomatic Influenza A Infection
Figure 6
Asymptomatic hosts showed unique temporal expression kinetics of cluster 6 genes related to JAK-STAT signaling transduction and protein biosynthesis.
(A,B) Distinct expression pattern of gene members in JAK-STAT pathway and their association with symptom severity. (A) Significant positive association between genes and disease severity is shown for 60 hpi (left); temporal gene expression pattern of suppressor of cytokine signaling 1 (SOCS1) shows upregulation in symptomatic hosts. (B) Significant negative association between genes and disease severity is shown for 60 hpi (left); temporal gene expression pattern of SOCS2 shows upregulation in Asx hosts versus downregulation in Sx hosts. (C) Significance of negative association (p-value) between ribosomal protein synthesis (RPS)-related genes and overall disease severity; Pie chart (top left) shows a high degree of enrichment of significant RPS genes in SOM cluster 6, which is characterized by a trend of upregulation (in Asx hosts) versus downregulation (in symptomatic hosts) over time. (D) Proportion overlap between cluster 6 ribosomal protein synthesis genes and lymphocyte signature ribosomal proteins genes [49].