Increased Susceptibility to Cortical Spreading Depression in the Mouse Model of Familial Hemiplegic Migraine Type 2
A. On the left, a sketch of the positioning of the stimulating (stim) and recording electrodes (1, 2) is outlined. Example of traces recorded in wild type (+/+) and Atp1a2+/R887 mutants (+/R887) at the two electrodes with dotted line indicating the wave recorded at the first electrode and full line indicating the wave recorded at the second electrode in both wild type and mutated animals. B. CSD threshold, velocity and duration of wild type and mutant are reported in box plots reporting bottom-up the 5th percentile, 25th percentile, median, 75th percentile and 95th percentile. The single dot represents the mean value described for each group. Atp1a2+/R887 heterozygous animals are more sensitive to CSD induction (+/R887, 13.00±1.7 µC, n = 20; +/+, 19.9±1.9 µC, n = 18; p<0.01; left graph). CSD velocity of propagation is increased in Atp1a2+/R887 mutants (wild type, 3.85±0.35 mm/min n = 18); mutant, 5.41±0.41 mm/min, n = 20; p<0.01; middle graph). No significant difference was observed in CSD duration (wild type 41.1±3.5 sec, n = 18; Atp1a2+/R887 40.1±3.19 sec, n = 20; p = 0.83; right graph).