FGF Signaling Regulates the Number of Posterior Taste Papillae by Controlling Progenitor Field Size
Figure 3
The CV progenitor field at E12.5 is expanded in Spry2−/− mice.
(A–H) Dorsal view of the expression of Sox2, Shh, Bmp7, and Wnt10b in wild-type and Spry2−/− littermates. Scale bar, 50 µm. (A'–H') Coronal sections of A–H. Scale bar, 50 µm. (I,J) Sagittal views of the CV placodes stained with E-cadherin show an expansion of the placode in the anterior(A)-posterior(P) orientation in Spry2−/− mice. Scale bar, 20 µm. (K) Quantification of the total number of cells in the CV placode. (L,M) Proliferating cells were detected by PCNA staining in coronal view. Scale bar, 20 µm. (N) The percentage of PCNA-positive cells in the CV placode at E12.5. * p<0.01. WT, wild-type; S2−/−, Spry2−/−.