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Quantitative Fitness Analysis Shows That NMD Proteins and Many Other Protein Complexes Suppress or Enhance Distinct Telomere Cap Defects

Figure 4

Confirmation of genetic interactions in an alternative genetic background.

A selection of genes identified by QFA were combined with either the yku70Δ or cdc13-1 mutations in the W303 genetic background and assessed for growth by manual spot test. Strains were cultured to saturation in 2 ml YPD at 23°C, a six-fold dilution series generated and spotted onto YPD. Strains were incubated at the indicated temperatures for three days before being photographed. All plates contained the reference strains 640 (wild type), 1108 (cdc13-1) and 1412 (yku70Δ), indicated as red cultures in the key. The “Wild Type” single mutant strains assessed were: 6656, 6811, 3622, 3653, 6620, 1273, 659, 6862, 6927 6951, 6963, 6692 and 6632. The cdc13-1 double mutant strains assessed were: 6810, 6814, 3624, 3655, 6614, 1296, 1258, 6860, 6928, 6865, 6967, 6694 and 6396. The yku70Δ double mutant strains assessed were: 6808, 6812, 4290, 4296, 6628, 1409, 1284, 2413, 2415, 6968, 6971, 6776 and 6763. Growth at other temperatures is shown in Figure S2.

Figure 4

doi: https://doi.org/10.1371/journal.pgen.1001362.g004