Quantitative Fitness Analysis Shows That NMD Proteins and Many Other Protein Complexes Suppress or Enhance Distinct Telomere Cap Defects
The yeast genome knock out collection was crossed to the yku70Δ mutation, or as a control to the ura3Δ mutation. 8 replicate crosses were performed and for each, the fitness of all double mutant cultures measured as in Figure 1. Growth of yku70Δ yfgΔ (“your favourite gene deletion”) double mutants was measured at 37.5°C and ura3Δ yfgΔ strains at 37°C. Gene deletions that significantly enhance (green) or suppress (red) the yku70Δ defect, in comparison with the ura3Δ mutation are indicated. Those marked by open circles have p-values <0.05 and those filled circles have FDR corrected p-values (q-values) <0.05. The line of equal growth (dashed grey) and a population model of expected fitness (solid grey) are indicated. The average position of his3Δ strains are indicated by solid light-blue lines on each axis, as proxy for “wild-type” growth.