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Quantitative Fitness Analysis Shows That NMD Proteins and Many Other Protein Complexes Suppress or Enhance Distinct Telomere Cap Defects

Figure 1

Cell fitness determination from growth on agar plates for quantitative fitness analysis (QFA).

A) Time course images of eight independent upf2Δ ura3Δ, yku70Δ ura3Δ and upf2Δ yku70Δ strains at the indicated temperatures; B) Cell density of individual replicate cultures was determined after image-analysis. The logistic growth model is fitted to each culture density time-series. The same data are plotted on linear or logarithmic scales on left and right respectively. C) Average values for Maximum Doubling Rate, Maximum Doubling Potential and Fitness (MDR, MDP and F respectively; see Text S1, experimental procedures), determined from the fitted curves. Data for yku70Δ ura3Δ is presented here to illustrate epistasis between yku70Δ and upf3Δ, however this is not how epistasis was calculated (see Figure 2 and Text S1, experimental procedures).

Figure 1