New Functions of Ctf18-RFC in Preserving Genome Stability outside Its Role in Sister Chromatid Cohesion
Figure 4
Cell cycle distribution and morphological abnormalities of dcc1 and ctf18 mutants.
(A) Quantification of cell morphology in log phase cultures. Several hundred cells (range 227–723) were scored for each genotype. Note that the dcc1Δ long tract was a mixture of cells with 155 repeats and contracted tracts by the end of the experiment; we were not able to complete a ctf18 long tract experiment without substantial contractions. Differences in the percentage of multi-budded cells were analyzed by a pooled variance t test using the Systat program; *, p<0.05, **, p<0.01 compared to wild type of the same tract length; ∧, p<0.05 compared to the no tract control of the same strain (e.g. p = 0.054 for dcc1-70 compared to dcc1 no tract, and p = 0.013 for ctf18-70 compared to ctf18 no tract). (B) Microscopic images of cells; all images are at the same scale, dcc1 and ctf18 mutants are characterized by an increase in cell size and the formation of protruded and multiple buds. Dotted lines indicate an overlay of another image to provide additional examples of cells of that genotype.