A Single cis Element Maintains Repression of the Key Developmental Regulator Gata2
Figure 1
Generation of mice lacking the −1.8 kb site of the Gata2 locus.
Diagram of GATA binding sites involved in the regulation of hematopoietic expression of Gata2 from its two alternate first exons, 1S and 1G, and their functional categories as defined by GATA-factor binding in G1E cells expressing only GATA2 and G1ER cells expressing only GATA1 (A). The region of the Gata2 locus targeted in generation of mouse strain deficient for a palindromic GATA binding site 1.8 kb upstream of the Gata2 transcriptional start site, referred to as the Δ-1.8 allele (B). PCR genotyping of mice deficient for the −1.8 GATA-binding site and littermate controls (C).