A Wnt-Frz/Ror-Dsh Pathway Regulates Neurite Outgrowth in Caenorhabditis elegans
Figure 2
Mutants with RMED/V neurite outgrowth defect isolated from the genetic screen.
(A) RME neuron fluorescence images of animals with different genetic backgrounds. unc-30(ju54);juIs76 is treated as wild type (WT). RMED/V posterior neurite outgrowth is unaffected in the D/V guidance cue mutants unc-6(ev400) and slt-1(eh15) and the vulvaless mutant lin-11(n389). (B) Quantification analysis of mutant phenotypes shown in (A). The average relative neurite length in wild type is set as 100. Error bars represent SEM. (C) Phenotypes and list of the three classes of mutants isolated from the genetic screen. Most Class I mutant animals lack both RMED and RMEV processes. Class II mutants display both shortened and normal length of neurites. In Class III mutants, both RMED and RMEV processes are short. Asterisk indicates the end of the process.