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A Comprehensive Genome-Wide Map of Autonomously Replicating Sequences in a Naive Genome

Figure 6

KARS12 and KARS101, two previously identified KlARSs.

(A) Overlapping ACSs of KARS12 (KlARS503) as predicted previously [29], [30] (colored in green) and in this study (colored in blue). Substitution mutations are shown in lower case font and deletions in dashes. Mutations that disrupt ARS function are highlighted in red, and mutations that do not affect ARS function are highlighted in black. The logo of 50bp ACS is superimposed for reference. (+) indicates ARS function and (−) indicates no ARS function. (B) Complete overlapping ACSs (colored in blue) of KARS101 identified by linker substitution analysis [28] and by motif search in this study. Octanucleotide linker mutations that destroyed ARS function (−) are indicated by red bars and those that compromised ARS function (+/−) are indicated by grey bars. The logo of 50bp ACS is superimposed for reference.

Figure 6

doi: https://doi.org/10.1371/journal.pgen.1000946.g006