Involvement of Global Genome Repair, Transcription Coupled Repair, and Chromatin Remodeling in UV DNA Damage Response Changes during Development
Figure 2
Germ cell and embryo survival following UV irradiation.
The percentages (survival) of hatched eggs following UVB irradiation are plotted against the indicated applied UVB-doses on germ cells in young adult animals carrying mutations in general NER factors (A and B), in rad-23 and csb-1 single and double mutants (C) and in xpc-1 single and rad-23; xpc-1 and xpc-1; csb-1 double mutants (D). (E) shows a simplified model of GGR and TCR in the germ line of C. elegans. Data for xpf-1 and ercc-1 in (B) were normalized because these mutants produce ∼20–25% unviable eggs without UV irradiation. Each line represents the mean of two or more independent experiments (typically, n>40 eggs). However, for xpa-1, xpg-1, xpf-1, ercc-1, rad-23; csb-1 and xpc-1; csb-1 mutants high UV doses severely decreased the amount of eggs laid. Survival was scored as zero if no eggs were laid. Error bars denote the s.e.m.