MicroRNA–Directed siRNA Biogenesis in Caenorhabditis elegans
Figure 1
Overview of the small RNAs found in HA::ALG-1, HA::ALG-2, and HA::RDE-1 immunoprecipitate libraries.
(A) Composition of small RNAs found in HA::ALG-1, HA::ALG-2, and HA::RDE-1 immunoprecipitates compared to those found in a library made of total small RNAs [18]. Small RNAs were divided in the twelve classes listed beside the graph. (B) A subset of miRNAs is enriched in HA::RDE-1 immunoprecipitates. In order to visualize the enrichment or depletion of miRNAs bound by RDE-1 versus ALG-1 as a function of total miRNA abundance (as defined by the abundance in ALG-1), the Log2 of the ratio of miRNA reads found in the HA::RDE-1 and HA::ALG-1 immunoprecipitate libraries was plotted against the Log2 of reads found in the HA::ALG-1 library. MiRNAs plotted left of the Y axis are depleted on RDE-1 immunoprecipitates, while those on the right are enriched in RDE-1.