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Down-Regulation of Honey Bee IRS Gene Biases Behavior toward Food Rich in Protein

Figure 3

Verification of IRS RNAi in peripheral fat body by whole-mount in situ hybridization.

Expression of IRS was confirmed in fat body, and staining intensity (purple/red color) was reduced in IRS knockdowns compared to controls. (A,B) High pollen-hoarding strain; (C,D) low pollen-hoarding strain; (E,F) wild type. The honey bee fat body is a single cell-layer primary composed of trophocytes (T) and oenocytes (O). IRS transcript was localized to both cell types. Magnification: 200×.

Figure 3