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Down-Regulation of Honey Bee IRS Gene Biases Behavior toward Food Rich in Protein

Figure 2

qRT–PCR confirms absence of IRS RNAi in brain.

IRS dsRNA injections did not affect IRS gene expression in the brain of high and low pollen-hoarding strains or wild-type worker bees. (A) Comparison of high and low pollen-hoarding strains showed that high strain workers had higher IRS expression levels in brain than low strain bees. (B) Wild type. Asterisks indicate significance, p<0.05. Bars are means ± s.e. Sample sizes are given inside bars. (A) versus (B) were quantified with different calibrator samples; Y-axes cannot be directly compared.

Figure 2