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Phosphorylation of the Conserved Transcription Factor ATF-7 by PMK-1 p38 MAPK Regulates Innate Immunity in Caenorhabditis elegans

Figure 6

Phosphorylation of ATF-7 by PMK-1.

(A) Cos7 cells were transfected with T7-ATF-7 or T7-ATF-7 carrying the P58S qd22 mutation, along with HA-PMK-1 and FLAG-SEK-1 as indicated. Whole cell extracts were immunoblotted with antibodies that recognize T7 (top), HA (middle), and FLAG (bottom). (B) Cos7 cells were transfected with T7-ATF-7 or T7-ATF-7 carrying the P58S qd22 mutation, along with kinase-dead (KD) HA-PMK-1 and FLAG-SEK-1 as indicated. ATF-7 was immunoprecipitated with anti-T7 and immunoblotted with anti-HA (top). Whole cell extract were immunoblotted with antibodies that recognize T7 (middle top), HA (middle bottom), and FLAG (bottom).

Figure 6

doi: https://doi.org/10.1371/journal.pgen.1000892.g006