Phosphorylation of the Conserved Transcription Factor ATF-7 by PMK-1 p38 MAPK Regulates Innate Immunity in Caenorhabditis elegans
Fluorescence and DIC microscopy of a representative L4-staged wild-type worm carrying an atf-7::GFP transgene under the regulation of the endogenous genomic atf-7 promoter and 3′-untranslated region. The red fluorescence from the pharynx is due to a Pmyo-2::RFP co-transformation marker. The rescuing capability of this translational fusion transgene was confirmed in both the atf-7(qd22 qd130) mutant (Figure S5) and atf-7(qd22 qd130); pmk-1(km25) double mutant (Figure S7). Scale bar, 100 µm.