Phosphorylation of the Conserved Transcription Factor ATF-7 by PMK-1 p38 MAPK Regulates Innate Immunity in Caenorhabditis elegans
qRT–PCR analysis of the expression of PMK-1-regulated genes. (A) L4 larval stage worms of the indicated genotype were propagated on E. coli OP50 and RNA was prepared as described in the Methods. Expression is relative to wild-type. Shown is the mean from two independent biological replicates with error bars representing SEM. (B) As in (A), except that worms of the indicated geneotype were exposed to P. aeruginosa PA14 for 4 h. Expression is relative to wild-type on E. coli OP50.