Advertisement

< Back to Article

Phosphorylation of the Conserved Transcription Factor ATF-7 by PMK-1 p38 MAPK Regulates Innate Immunity in Caenorhabditis elegans

Figure 3

The loss-of-function atf-7(qd22 qd130) mutation suppresses the immunodeficient phenotype caused by deficient signaling in the PMK-1 pathway.

(A) Fluorescence microscopy images of GFP expression from the agIs219 transgene in pmk-1(km25) and atf-7(qd22 qd130); pmk-1(km25) one-day-old adults. (B) Pathogenesis assay of L4 larval stage wild-type worms; atf-7(qd22 qd130) and pmk-1(km25) mutant animals; and atf-7(qd22 qd130); pmk-1(km25) double mutant animals, on P. aeruginosa PA14. All strains carry the agIs219 transgene. The difference in susceptibility between pmk-1(km25) mutant animals and atf-7(qd22 qd130); pmk-1(km25) double mutant animals is significant (p<0.0001). Replicate data can be seen in Figure S14. (C) Fluorescence microscopy images of GFP expression from the agIs219 transgene in sek-1(km4) and atf-7(qd22 qd130); sek-1(km4) one-day-old adults. (D) Pathogenesis assay of L4 larval stage wild-type worms; atf-7(qd22 qd130) and sek-1(km4) mutant animals; and atf-7(qd22 qd130); sek-1(km4) double mutant animals, on P. aeruginosa PA14. All strains except for KU25 [sek-1(km4)] carry the agIs219 transgene. The difference in susceptibility between sek-1(km4) mutant animals and atf-7(qd22 qd130); sek-1(km4) double mutant animals is significant (p<0.0001). Replicate data can be seen in Figure S15.

Figure 3

doi: https://doi.org/10.1371/journal.pgen.1000892.g003