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A Phenotypic Profile of the Candida albicans Regulatory Network

Figure 3

A model of the differences in iron homeostasis regulation between S. cerevisiae and C. albicans.

(A) Each ellipse is colored to reflect whether the indicated gene(s) are believed to play a role in iron homeostasis in C. albicans (blue), S. cerevisiae (burgundy), or both species (green). (B) C. albicans phenotyping data that informed the model are displayed in the bottom panel. These phenotypes include sensitivity to the iron chelator BPS, copper-sensitivity/resistance, and sensitivity to alkaline pH. For each growth condition, only mutants with strong phenotypes are shown (ΔΔmac1 was not scored on alkaline medium due to poor growth on the YEPD base). Each square phenotype panel displays the wild-type phenotype (1× and 5× dilutions) above the mutant phenotype (the image coloration is inverted to better highlight growth). The panels display the timepoint and medium concentration that showed the most dramatic difference between wild-type and mutant. BPS phenotypes were also assayed for S. cerevisiae mutants, and were identical to those of the C. albicans orthologs, with two exceptions: (1) the CCAAT-complex mutants were BPS-sensitive in C. albicans, but not S. cerevisiae, and (2) Δaft1 and Δaft2 were BPS-sensitive in S. cerevisiae but the likely C. albicans ortholog (ΔΔorf19.2272) was not BPS-sensitive. Although the C. albicans ΔΔcsr1 mutant is BPS-sensitive, we have omitted Csr1 because the phenotype is only evident at high BPS concentrations and is likely to reflect a role in zinc homeostasis (see BPS entry in Text S1).

Figure 3